Try using Presets first. Choose a preset depending on the pattern of your images. If your image has low contrast or weak fluorescence, click Low Contrast or Weak Fluorescence radio buttons, respectively. If the image is average contrast and fluorescence, use the default Average preset. There is no need to reset the image if you wish to try an another preset.
Remember to use background correction settings consistently when analyzing images in the same study.
If you wish to analyze colocalization in a specific area of the image, choose the ROI tool that will enable the most precise selection of the area you will be examining. Lasso or Polygon serve best for this task.
To open an image quickly, drag and drop it onto the CoLocalizer Pro icon - just hold down the Command-Option keys while you drag. You can drop image onto any CoLocalizer Pro icon, whether it is in the Dock, in the Finder's sidebar, or in the Finder itself.
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