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Pixel-based vs object-based approach to quantify colocalization: which one to choose?


The currently used methods to quantify colocalization in fluorescence microscopy images determine its degree my measuring the overlap of pixel intensities in different color channels and are therefore essentially pixel-based. The biggest issue with pixel-based methods is their notorious dependence on background noise in the images. While this noise can be minimized to a significant degree using specialized software tools, it can still make an impact when using coefficients sensitive to it.

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