Use Smart Background Correction. This brand new tool ties together background correction and ROI selection into a single step. You will be able to correct background specifically for the selected ROI considering its unique properties.
Remember to use background correction settings consistently when analyzing images in the same study.
If you wish to analyze colocalization in a specific area of the image, choose the ROI tool that will enable the most precise selection of the area you will be examining. Lasso or Polygon serve best for this task.
To open an image quickly, drag and drop it onto the CoLocalizer Pro icon - just hold down the Command-Option keys while you drag. You can drop image onto any CoLocalizer Pro icon, whether it is in the Dock, in the Finder's sidebar, or in the Finder itself.
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